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Abstract: Detection of singlet oxygen production for PDT treatments
both in vitro and in vivo using a diode laser-based
singlet oxygen monitor
S. Lee, D.H. Vu, M.F. Hinds, S.J. Davis, T. Hasan, A. Khachemoune, W. Rice, N.R. Sznycer-Taub,
"Detection of singlet oxygen production for PDT treatments
both in vitro and in vivo using a diode laser-based
singlet oxygen monitor
,"
presented at Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV Biomedical Optics (BiOS) Symposium
(San Jose, CA)
,
(21-26 January2006).
Copyright © 2006 Society of Photo-Optical Instrumentation Engineers.
This paper was published in Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XV Biomedical Optics (BiOS) Symposium,
and is made available as an electronic reprint (preprint)
with permission of SPIE. One print or electronic copy may be made for personal use only. Systematic or multiple
reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this
paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Abstract
Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses photosensitizers that are selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in their metastable singlet delta state, O2(1Ä), are believed to be the species that destroys cancerous cells during PDT. PSI has developed a fiber optic-coupled, diode laser- based diagnostic for singlet molecular oxygen produced during PDT. This portable prototype system detects the singlet oxygen emission near 1.27 µm. We have obtained signals from singlet oxygen during and
following pulsed laser excitation and demonstrated excellent sensitivity with this system. Both in vitro and in vivo studies have been completed, and we have detected the production of singlet oxygen during PDT treatments with both animal and human subjects. Effects of photobleaching have also been observed. These results are promising for the development of the sensor as a real-time dosimeter for PDT.
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